In this study, the sucrose phosphorylase gene 1355SPase was cloned by PCR amplification from the chromosomal DNA of Leuconostoc mesenteriodes NRRLB-1355. The sequence analysis showed that this gene was composed of 1517 bp nucleotides, with one major open reading frame of 1494 bp encoding a polypeptide of 497 amino acid residues. The amino acid sequences of this gene are almost identical except two residues, to those genes previously reported from other bacteria. The purified 1355SPase showed an apparent MW of 56.6 kDa and specific activity of 2 U mg -1 for sucrose. The effects of temperature and pH on the enzyme activity were also determined. The optimum temperature and pH were 37°C and 6.7, respectively. To our knowledge, this is the first study reporting the activity and the full length sequence of 1355SPase.